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Expression of a Disintegrin and Metalloprotease 10 Gene Polymorphisms in a Cohort of Egyptian Patients with Hepatocellular Carcinoma

[ Vol. 17 , Issue. 4 ]

Author(s):

Amal A. Mohamed , Dina M. Abo-Elmatty , Omnia I ezzat , Ahmed A. Youssef , Eman T. Mehanna , Alshymaa A. Hassnine , Noha M. Mesbah, Salma Saed, Eman Al Sayed , Mahmoud Hamada , Afaf F. Khamis, Ayman Elshentenawy, Marwa S.E. Abd El-Raouf, Sherief Abd-Elsalam* and Amr M. Elsayed   Pages 312 - 318 ( 7 )

Abstract:


Background: Hepatocellular carcinoma (HCC) is the third leading cause of cancer mortality. There is a need for a marker associated with HCC progression. A disintegrin and metalloprotease (ADAMs) family proteins have a lot of functions in cell adhesion, migration, proteolysis and signaling.

Aims: The aim of the study was to investigate the relation between ADAM 10 gene single nucleotide polymorphisms (SNPs) and HCC progression.

Methods: This study involved 201 cases divided: Group I (67 HCC patients), Group II (67 cirrhotic patients), Group III (67 control). Each group was subjected to laboratory investigations: (CBC, blood sugar, kidney and liver function, viral markers, alpha fetoprotein), imaging: (abdominal ultrasonography, and triphasic C.T) and ADAM 10 gene polymorphism (rs 653765, rs 383902) detection by real – time PCR.

Results: There was a statistically significant difference in the frequency and genotyping of ADAM10 SNPs in HCC patients in comparison to cirrhotic and control groups [the frequency of rs 653765 genotypes (p=0.015) and model (p=0.013)]; likewise, the frequency of rs 383902 genotypes (p<0.001) and model (p=0.001)). Also, there was a statistically significant association between different SNP rs 383902 genotype with CLIP stages (p=0.02) and with VISUM stages (p=0.035).

Conclusion: ADAM-10 is overexpressed in HCC patients and involved in HCC progress. These findings highlight that ADAM inhibitor may be used as therapeutic goals in the treatment of HCC.

Keywords:

Hepatocellular carcinoma (HCC), adisintegrin and metalloprotease (ADAMs), liver cirrhosis, single nucleotide polymorphisms (SNP), HBV, HCV.

Affiliation:

Department of Biochemistry, National Hepatology and Tropical Medicine Research Institute, Cairo, 176, Department of Biochemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Department of Biochemistry, Faculty of Pharmacy, Egyptian Russian University, Bade City, Department of Biochemistry, Faculty of Pharmacy, Egyptian Russian University, Bade City, Department of Biochemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Department of Gasteroentrology and Tropical Medicine, Faculty of Medicine, Minia University, Minia, Department of Biochemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, Clinical Pathology Department, Faculty of Medicine, Minia University, Minia, Department of Internal Medicine, Faculty of Medicine, Benha University, Benha, Clinical Pathology Department, Faculty of Medicine, Benha University, Benha, Kasr Al-Ainy Center of Clinical Oncology and Nuclear Medicine (NEMROCK), Kasr Al-Ainy School of Medicine, Cairo University, Cairo, Department of Community, Environmental and Occupational Medicine, Faculty of Medicine, Benha University, Benha, Tropical Medicine Department, Tanta University, Tanta, Department of Gasteroentrology and Tropical Medicine, Faculty of Medicine, Minia University, Minia



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